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genechip expression analysis technical manual  (Thermo Fisher)


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    Thermo Fisher genechip expression analysis technical manual
    Genechip Expression Analysis Technical Manual, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genechip+expression+analysis+technical+manual/pm37668368-272-17-22
    Average 90 stars, based on 1 article reviews
    genechip expression analysis technical manual - by Bioz Stars, 2026-10
    90/100 stars

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    Expressing:

    Article Title: The Transcriptional Repressor TupA in Aspergillus niger Is Involved in Controlling Gene Expression Related to Cell Wall Biosynthesis, Development, and Nitrogen Source Availability
    Article Snippet: .. Probe synthesis and fragmentation were performed at ServiceXS (Leiden, Netherlands) according to the GeneChip Expression Analysis Technical Manual (Affymetrix Inc. 2002). .. DSM (Delft, Netherlands) proprietary A. niger gene chips were hybridized, washed, stained, and scanned as described in the Gene-Chip Expression Analysis Technical Manual (Affymetrix Inc. 2002).

    Article Title: Use of ICOS-based cars to enhance antitumor activity and car persistence
    Article Snippet: Microarray Target Preparation and Hybridization Microarray services were provided by the UPenn Microarray Facility, including quality control tests of the total RNA samples by Agilent Bioanalyzer and Nanodrop spectrophotometry. .. All protocols were conducted as described in the Affymetrix GeneChip Expression Analysis Technical Manual. .. Second-strand cDNA synthesis was followed by in vitro transcription with T7 RNA polymerase for linear amplification of each transcript, and the resulting cRNA was converted to cDNA, fragmented, assessed by Bioanalyzer, and biotinylated by terminal transferase end labeling. cRNA yields ranged from 36-89 ug, and cDNA was added to Affymetrix hybridization cocktails, heated at 99° C. for 5 min and hybridized for 16 h at 45° C. to Human Gene 1.0ST GeneChips (Affymetrix Inc., Santa Clara CA).

    Article Title: Dose-dependent effects of small-molecule antagonists on the genomic landscape of androgen receptor binding
    Article Snippet: Statistic analysis was performed using two-way ANOVA (GraphPad Prism, Version 5.01 - http://www.graphpad.com ). .. RNA from VCaP cells treated with AR-siRNA, Compound 30 (10 μM) or corresponding controls underwent 1st and 2nd strand cDNA synthesis, in vitro reverse transcription, and target preparation following the GeneChip Expression Analysis Technical Manual (Affymetrix). .. Overnight hybridization of the fragmented cRNA on the GeneChip® Human Genome U133A 2.0 array and subsequent washing, staining and scanning steps were performed as suggested by the manufacturer (Affymetrix).

    Article Title: Reactive oxygen species and transcript analysis upon excess light treatment in wild-type Arabidopsis thaliana vs a photosensitive mutant lacking zeaxanthin and lutein
    Article Snippet: RNA integrity was assessed using the Agilent RNA 6000 nano kit and Agilent Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA). .. RNA samples were processed following the Affymetrix GeneChip Expression Analysis Technical Manual (Affymetrix, Inc., Santa Clara, CA). ..

    Article Title: Molecular fingerprinting reflects different histotypes and brain region in low grade gliomas
    Article Snippet: Quantification and quality assurance were performed using the NanoDrop spectrophotometer (NanoDrop Technologies Wilmington, Delawere USA) and the Agilent 2100 bioanalyzer (Agilent Technologies, Waldbronn, Germany), respectively. .. Double-stranded cDNA were processed according to the Affymetrix GeneChip Expression Analysis Technical Manual (Affymetrix, Santa Clara, CA). .. Microarray data for 40 LGG samples was generated with Affymetrix HG-U133Plus2.0 arrays (Affymetrix, Santa Clara, CA).

    Article Title: Identification of a novel viral factor inducing tumorous symptoms by disturbing vascular development in planta .
    Article Snippet: RNA was amplified with Amplification Ovation RNA Amplification system V2 (NuGEN, San Carlos, CA, USA) and then purified using MinElute Reaction Cleanup kit (QIAGEN, Hilden, Germany). cRNA was biotinylated with GenChip One-Cycle Target Labeling and Control Reagents (Affymetrix). .. Hybridization, washing, and staining were conducted with Fluidics Station 450 (Thermo Fisher Scientific) as described in the GeneChip Expression Analysis Technical Manual (Thermo Fisher Scientific). .. Scanning was conducted with GeneChip Scanner 3000 7G (Thermo Fisher Scientific) as described in the Gene Chip Expression Analysis Technical Manual.

    Article Title: Performance evaluation of commercial short-oligonucleotide microarrays and the impact of noise in making cross-platform correlations
    Article Snippet: The labeled cRNA was then purified using an RNeasy mini kit (Qiagen) according to the manufacturer's protocol and ethanol precipitated. .. Fragmentation of cRNA, hybridization, washing, staining, and scanning were performed as described in the Affymetrix GeneChip Expression Analysis Technical Manual [ ]. ..

    Article Title: Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean
    Article Snippet: Samples were then hybridized to the Soy Genome Affymetrix ® GeneChip using an Affymetrix ® Hybridization Oven 640, and arrays were washed on an Affymetrix ® Fluidics Station 450 using Affymetrix ® fluidics protocol EukGE-WS2v4_450. .. Details of this protocol can be found in the Affymetrix ® Genechip Expression Analysis Technical Manual, Section 2, Chapter 3 http://www.affymetrix.com/support/downloads/manuals/expression_analysis_technical_manual.pdf . .. The Soy Genome Affymetrix ® GeneChip http://www.Affymetrix.com containing greater than 37,500 probesets and representing 35,611 soybean transcripts [ ], was used to assess gene expression.

    cDNA Synthesis:

    Article Title: Dose-dependent effects of small-molecule antagonists on the genomic landscape of androgen receptor binding
    Article Snippet: Statistic analysis was performed using two-way ANOVA (GraphPad Prism, Version 5.01 - http://www.graphpad.com ). .. RNA from VCaP cells treated with AR-siRNA, Compound 30 (10 μM) or corresponding controls underwent 1st and 2nd strand cDNA synthesis, in vitro reverse transcription, and target preparation following the GeneChip Expression Analysis Technical Manual (Affymetrix). .. Overnight hybridization of the fragmented cRNA on the GeneChip® Human Genome U133A 2.0 array and subsequent washing, staining and scanning steps were performed as suggested by the manufacturer (Affymetrix).

    In Vitro:

    Article Title: Dose-dependent effects of small-molecule antagonists on the genomic landscape of androgen receptor binding
    Article Snippet: Statistic analysis was performed using two-way ANOVA (GraphPad Prism, Version 5.01 - http://www.graphpad.com ). .. RNA from VCaP cells treated with AR-siRNA, Compound 30 (10 μM) or corresponding controls underwent 1st and 2nd strand cDNA synthesis, in vitro reverse transcription, and target preparation following the GeneChip Expression Analysis Technical Manual (Affymetrix). .. Overnight hybridization of the fragmented cRNA on the GeneChip® Human Genome U133A 2.0 array and subsequent washing, staining and scanning steps were performed as suggested by the manufacturer (Affymetrix).

    Reverse Transcription:

    Article Title: Dose-dependent effects of small-molecule antagonists on the genomic landscape of androgen receptor binding
    Article Snippet: Statistic analysis was performed using two-way ANOVA (GraphPad Prism, Version 5.01 - http://www.graphpad.com ). .. RNA from VCaP cells treated with AR-siRNA, Compound 30 (10 μM) or corresponding controls underwent 1st and 2nd strand cDNA synthesis, in vitro reverse transcription, and target preparation following the GeneChip Expression Analysis Technical Manual (Affymetrix). .. Overnight hybridization of the fragmented cRNA on the GeneChip® Human Genome U133A 2.0 array and subsequent washing, staining and scanning steps were performed as suggested by the manufacturer (Affymetrix).

    Hybridization:

    Article Title: Identification of a novel viral factor inducing tumorous symptoms by disturbing vascular development in planta .
    Article Snippet: RNA was amplified with Amplification Ovation RNA Amplification system V2 (NuGEN, San Carlos, CA, USA) and then purified using MinElute Reaction Cleanup kit (QIAGEN, Hilden, Germany). cRNA was biotinylated with GenChip One-Cycle Target Labeling and Control Reagents (Affymetrix). .. Hybridization, washing, and staining were conducted with Fluidics Station 450 (Thermo Fisher Scientific) as described in the GeneChip Expression Analysis Technical Manual (Thermo Fisher Scientific). .. Scanning was conducted with GeneChip Scanner 3000 7G (Thermo Fisher Scientific) as described in the Gene Chip Expression Analysis Technical Manual.

    Article Title: Performance evaluation of commercial short-oligonucleotide microarrays and the impact of noise in making cross-platform correlations
    Article Snippet: The labeled cRNA was then purified using an RNeasy mini kit (Qiagen) according to the manufacturer's protocol and ethanol precipitated. .. Fragmentation of cRNA, hybridization, washing, staining, and scanning were performed as described in the Affymetrix GeneChip Expression Analysis Technical Manual [ ]. ..

    Staining:

    Article Title: Identification of a novel viral factor inducing tumorous symptoms by disturbing vascular development in planta .
    Article Snippet: RNA was amplified with Amplification Ovation RNA Amplification system V2 (NuGEN, San Carlos, CA, USA) and then purified using MinElute Reaction Cleanup kit (QIAGEN, Hilden, Germany). cRNA was biotinylated with GenChip One-Cycle Target Labeling and Control Reagents (Affymetrix). .. Hybridization, washing, and staining were conducted with Fluidics Station 450 (Thermo Fisher Scientific) as described in the GeneChip Expression Analysis Technical Manual (Thermo Fisher Scientific). .. Scanning was conducted with GeneChip Scanner 3000 7G (Thermo Fisher Scientific) as described in the Gene Chip Expression Analysis Technical Manual.

    Article Title: Performance evaluation of commercial short-oligonucleotide microarrays and the impact of noise in making cross-platform correlations
    Article Snippet: The labeled cRNA was then purified using an RNeasy mini kit (Qiagen) according to the manufacturer's protocol and ethanol precipitated. .. Fragmentation of cRNA, hybridization, washing, staining, and scanning were performed as described in the Affymetrix GeneChip Expression Analysis Technical Manual [ ]. ..



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